Course: Biological Specimens in Electron Microscopes 2021

  • 25.2.2021
  • Kurzy

October, 4 - 8, 2021, Biology Centre of CAS, České Budějovice | COURSE WEBSITE


Description: 

This practical course is focused on the advanced methods of biological specimen preparation for electron microscopy. The main goal is to make participants familiar with the theoretical background and the latest practical developments in this field. The programme includes state of the art fixation, including cryo-methods, cutting of ultrathin sections from embedded or frozen samples, methods of negative staining and immunolabeling, specimen preparation for transmission and scanning electron microscopy and methods of specimen preparation for 3D electron microscopy (single particle analysis, electron tomography, array tomography and serial block-face). The results will be evaluated using electron microscopes. The course is limited to 15 participants. It is intended for Master and PhD students, young researchers, technicians, and new EM users. This course is supported by the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project “National Infrastructure for Biological and Medical Imaging (Czech-BioImaging –LM2018129)“.

Invited speakers:

Vladislav Krzyžánek (Institute of Scientific Instruments CAS, Brno)

Group leader of Microscopy of Biomedicine focusing on quantitative imaging by STEM (mass-thickness measurement by electron scattering), low-temperature scanning electron microscopy (cryo-SEM), correlation of signals in electron microscopy.

Oldřich Benada (Institute of Microbiology CAS, Prague)

Head of the EM group. He is generally interested in electron microscopy in microbiology (TEM, SEM, STEM) and visualisation of the macromolecules and macromolecular complexes.

Jiří Týč (Laboratory of EM, Biology Centre CAS, České Budějovice)

His main research interest is BIOIMAGING. He got his PhD in molecular biology, and during his postdoc at the Bioimaging unit at Oxford Brookes University (UK), he started with 3D EM. He focuses on electron microscopy, but he has experience also with confocal and high-resolution fluorescence microscopy. He specialises in 3D SEM techniques such as SBF-SEM (serial block-face scanning electron microscopy) and array tomography.

Zdeno Gardian (Macrocomplex group, South Bohemia University & Biology Centre of the CAS, České Budějovice)

His research interests are single-particle and image analysis, structural studies of photosynthetic reaction centres and light-harvesting complexes. Information Programme Registration and Contact Venue and Accommodation

Tomáš Bílý (Macrocomplex group, South Bohemia University & Biology Centre of the CAS, České Budějovice)

His research interests are electron tomography, image processing, 3D visualisation of cells, purified organelles and viruses. 

Instructors: Mgr. František Kitzberger, Mgr. Martina Tesařová, Mgr. Eva Ďurinová Petra Masařová, Jiří Vaněček

Official website: CLICK HERE

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